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Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)
引用本文:PAN Junsong*,WANG Gang*,LI Xiaozun,HE Huanle,WU Aizhong and CAI Run**(Agriculture and Biology School,Shanghai Jiaotong University,Shanghai 201101,China). Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber (Cucumis sativus L.)[J]. 自然科学进展(英文版), 2005, 15(5). DOI: 10.1080/10020070512331342310
作者姓名:PAN Junsong*  WANG Gang*  LI Xiaozun  HE Huanle  WU Aizhong and CAI Run**(Agriculture and Biology School  Shanghai Jiaotong University  Shanghai 201101  China)
作者单位:Agriculture and Biology School,Shanghai Jiaotong University,Shanghai 201101,China
基金项目:上海市科委资助项目,Shanghai Municipal Agricultural Commission
摘    要:Cucumber (Cucumis sativus L.) is an annualclimber originated from the tropic rain forest area inthe south of Himalayas, which belongs to the Cucur bitaceae family. Cucumber is one of the importantvegetables in the world, and its planting area is sec ond only to that of tomato[1]. However genetics re search on cucumber obviously drops behind that of thelatter. The classic genetic map of cucumber, with sixlinkage groups, is composed of more than 100 genesfor color, morphology, and dise…


Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber ( Cucumis sativus L. )
PAN Junsong,WANG Gang,LI Xiaozun,He Huanle,WU Aizhong,CAI Run. Construction of a genetic map with SRAP markers and localization of the gene responsible for the first-flower-node trait in cucumber ( Cucumis sativus L. )[J]. Progress in Natural Science, 2005, 15(5). DOI: 10.1080/10020070512331342310
Authors:PAN Junsong  WANG Gang  LI Xiaozun  He Huanle  WU Aizhong  CAI Run
Abstract:With an F2 population from the cross of two cucumber inbred lines, S06 and S52, sequence-related amplified polymorphism (SRAP) was used to construct a genetic linkage map in cucumber (Cucumis sativus L. ). Sixty-four SRAP primer combinations generated 108 polymorphic bands in the F2 population analysis. The average of polymorphic bands produced by one primer pair was 1.5,and the maximum was 5. Using Mapmaker 3.0, a linkage map was constructed, which consisted of 77 SRAP markers distributed in nine linkage groups (LOD≥3.0) and spanned 1114.2 cM with an average interval of 14.5 cM between markers. The gene for the first-flowernode trait, termed ffn, was mapped to linkage group IX, flanked by DC1EM5 and ME7EM2A at 10.3 cM and 12.1 cM distance, respectively.
Keywords:cucumber ( Cucumis sativus L. )  SRAP (sequence-related amplified polymorphism)  linkage map  first flower node
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