| 罗非鱼无乳链球菌LAMP快速检测方法的建立 |
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| 引用本文： | 郑 磊,樊海平,吴 斌,曾占壮,钟全福,张新艳.罗非鱼无乳链球菌LAMP快速检测方法的建立[J].福州大学学报(自然科学版),2015,43(4):572-576. |
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| 作者姓名： | 郑 磊 樊海平 吴 斌 曾占壮 钟全福 张新艳 |
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| 作者单位： | 福建省淡水水产研究所,福建省淡水水产研究所,福建省淡水水产研究所,福建省淡水水产研究所,福建省淡水水产研究所,福建省淡水水产研究所 |
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| 基金项目： | 福建省科技厅公益类科研院所专项（2012R002-1），现代农业产业技术体系建设专项基金（CARS-46-35，CARS-39）资助 |
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| 摘 要： | 针对无乳链球菌高度保守的表面免疫相关蛋白基因,采用环介导等温扩增技术(LAMP),设计4条特异性引物,优化反应体系,建立罗非鱼无乳链球菌快速诊断方法.研究结果表明,LAMP方法检测灵敏度达到30 CFU·m L-1,且对2株罗非鱼源无乳链球菌均能扩增出特异性片段,而对14株非无乳链球菌均未扩增出相应片段.建立的LAMP检测方法具有高度的特异性和灵敏性,反应在1 h内完成,为罗非鱼无乳链球菌病的快速诊断提供了一种重要的技术手段.
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| 关 键 词： | LAMP 无乳链球菌 sip 检测 |
| 修稿时间： | 8/8/2014 12:00:00 AM |
Development of loop-mediated isothermal amplification for detection of Streptococcus agalactiae from Tilapia |
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| ZHENG Lei,FAN Haiping,WU Bin,ZENG Zhanzhuang,ZHONG Quanfu and ZHANG Xinyan.Development of loop-mediated isothermal amplification for detection of Streptococcus agalactiae from Tilapia[J].Journal of Fuzhou University(Natural Science Edition),2015,43(4):572-576. |
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| Authors: | ZHENG Lei FAN Haiping WU Bin ZENG Zhanzhuang ZHONG Quanfu and ZHANG Xinyan |
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| Institution: | Freshwater Fisheries Research Institute of Fujian Province,Freshwater Fisheries Research Institute of Fujian Province,Freshwater Fisheries Research Institute of Fujian Province,Freshwater Fisheries Research Institute of Fujian Province,Freshwater Fisheries Research Institute of Fujian Province,Freshwater Fisheries Research Institute of Fujian Province |
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| Abstract: | To develop a technique for detecting the surface immunogeenic protein(sip) gene of Streptococcus agalactiae using a DNA amplification method designated loop-mediated isothermal amplification (LAMP) . The rapid detection method was established by using optimum reaction system with four specially primers of the target. Results showed that the method had perfect specificity,as 2 strains of Streptococcus agalactiae were able to amplify specific fragments,while 14 strains of non- Streptococcus agalactiae were not.The amplification can be obtained in 1 hour with the sensitivity low to 30 cfu/ml.The established method povides a better choice for rapid detection of Streptococcus agalactiae. |
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| Keywords: | LAMP Streptococcus agalactiae sip detection |
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