Fusion polypeptides in gene cloning: Potential problems due to conformational alterations at the junction |
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Authors: | E. Querol A. Parrilla |
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Affiliation: | (1) Instituto de Biología Fundamental V. Villar-Palasí and Departmento de Bioquímica, Universidad Autónoma de Barcelona, 08193 Bellaterra, Barcelona, (Spain) |
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Abstract: | Summary Many eukaryotic genes are cloned in bacterial hosts as fusion polypeptides. Prediction of the secondary structures for some common prokaryotic fusion polypeptides shows that many junction sites correspond to important secondary structures. It is suggested that such structures could affect (hinder, etc.) the conformation or drive the folding of the neighboring eukaryotic counterparts. Thus the prokaryotic junction should be better performed, in random coil regions, or short fusion prokaryotic polypeptides should be sued.1 October 1986 |
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Keywords: | Molecular cloning fusion polypeptides protein conformation protein folding |
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