| 四膜虫乳酸、乳酸脱氢酶同工酶及培养液溶解氧的研究 |
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| 引用本文： | 沈锡祺,候连生,张作人.四膜虫乳酸、乳酸脱氢酶同工酶及培养液溶解氧的研究[J].上海师范大学学报(自然科学版),1988(1). |
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| 作者姓名： | 沈锡祺 候连生 张作人 |
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| 作者单位： | 华东师范大学生物系，华东师范大学生物系，华东师范大学生物系 |
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| 摘 要： | 原生动物四膜虫 Tetrahymena shanghaiensis Sl 是一种好氧性生物,但是在少氧甚至缺氧的培养液里能够存活而且存活相当长的时间。在不同的生理状态下生长的四膜虫,经测定其培养液中溶解氧的含量是不同的,在四膜虫生长的前对数期时,其培养液里溶解氧已有很大减少,长期保持和衰老期的四膜虫溶液中的溶解氧接近零或非常少;四膜虫乳酸的含量也有差别,长期保持溶液中的四膜虫细胞乳酸含量要比衰老期、对数期都大;四膜虫乳酸脱氢酶同工酶经聚丙烯酰胺凝胶电泳分析,表明在不同状态下该酶蛋白带有明显差别,主要反映在对数生长期细胞的蛋白带不同于衰老期、长期保持的细胞所具有的蛋白带。
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| 关 键 词： | 四膜虫 溶解氧 乳酸含量 乳酸脱氢酶同工酶 |
A Study on Lactate,LDH Isoenzyme and Dissolved O_2 in Tetrahyrnena shanghaiensis SI |
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| SHEN XIQI HOU LIANSHENG ZHANG ZUOREN.A Study on Lactate,LDH Isoenzyme and Dissolved O_2 in Tetrahyrnena shanghaiensis SI[J].Journal of Shanghai Normal University(Natural Sciences),1988(1). |
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| Authors: | SHEN XIQI HOU LIANSHENG ZHANG ZUOREN |
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| Institution: | SHEN XIQI HOU LIANSHENG ZHANG ZUOREN (TCHANG TSOREN) (Department of Biology) |
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| Abstract: | Tetrahymena shanghaiensis Sl is an aerobic organism,but it can grow well for long time in the medium with low O_2 concentration,even in absence of O_2.The content of dissolved O_2 is very different in the medium of growing T.Sl under different physiological conditions. We have studied LDH isoenzyme of T.Sl in different physiological conditions. It is obvious that cell in long-term maintenance medium contains higher level of LDH_1,LDH_2,LDH_3,LDH_5.Cell in dead phase consists of LDH_1,LDH_2,LDH_3 at 70 hr,but consists of LDH_5 only at 90hr.Cell in log.phase contains higher level of LDH_2,in adition to,LDH_1,LDH_5 visible on the gel. |
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| Keywords: | Tetrahymena shanghaiensis S1 dissolved O_2 lactate content LDH isoenzyme |
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