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Mitochondrial DNA variation in geographic populations of Colorado potato beetle,Leptinotarsa decemlineata (Coleoptera; Chrysomelidae) |
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| Authors: | A M L Azeredo-Espin R F W Schroder M D Huettel W S Sheppard |
| Institution: | (1) Insect Biocontrol Laboratory, ARS-USDA, Bldg 011-A, Barc-West, 20705 Beltsville, Maryland, USA;(2) Bee Research Laboratory, ARS-USDA, Bldg 476, Barc-East, 20705 Beltsville, Maryland, USA;(3) Present address: Depto de Genetica e Evolucao, Universidade Estadual de Campinas, IB-UNICAMP, Brasil |
| Abstract: | Summary This study demonstrates variability in restriction enzyme cleavage sites of mitochondrial DNA (mtDNA) among four popalations of Colorado potato beetle (CPB). A suite of three enzymes (EcoRI,HpaI,PstI) was sufficient to discriminate among the populations tested. Individuals heteroplasmic for restriction enzyme patterns were found in some populations. Variability in CPB mtDNA should prove useful in efforts to trace the origin and dispersal of the species in North America. |
| Keywords: | Mitochondrial DNA RFLP Leptinotarsa decemlineata Colorado potato beetle population genetics |
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