| 丁酸钠对重组CHO细胞生长及产物EPO表达的影响 |
| |
| 引用本文： | 周玲,孙祥明,张元兴.丁酸钠对重组CHO细胞生长及产物EPO表达的影响[J].华东理工大学学报(自然科学版),2001,27(3):310-312,319. |
| |
| 作者姓名： | 周玲 孙祥明 张元兴 |
| |
| 作者单位： | 华东理工大学 |
| |
| 摘 要： | 在CHO－S－SFM II无血清培养基中维持培养重组CHO细胞，考查添加丁酸钠对细胞生长，糖代谢及产物EPO表达的影响，丁酸钠浓度达到2mmol/L时完全抑制了细胞的生长，同时，丁酸钠的添加也降低了葡萄糖比消耗速率（30％左右），在本培养系统中，丁酸钠并不能促进产物的表达，但有助于维持EPO表达的稳定性。
|
| 关 键 词： | 丁酸钠 CHO细胞 重组人促红细胞生成素 细胞培养 EPO表达 细胞生长 糖代谢 |
| 文章编号： | 1006-3080(2001)03-0310-03 |
Effect of Butyrate on Cell Growth and Expression of EPO in Recombinant CHO Cells |
| |
| ZHOU Ling,SUN Xiang\|ming,ZHANG Yuan\|xing.Effect of Butyrate on Cell Growth and Expression of EPO in Recombinant CHO Cells[J].Journal of East China University of Science and Technology,2001,27(3):310-312,319. |
| |
| Authors: | ZHOU Ling SUN Xiang\|ming ZHANG Yuan\|xing |
| |
| Institution: | ZHOU Ling,SUN Xiang\|ming,ZHANG Yuan\|xing * |
| |
| Abstract: | Recombinant CHO cells were cultured in CHO\|S\|SFM II.The effects of sodium butyrate on cell growth,glucose metabolism and EPO expression were investigated.Cell growth decreased with the addition of sodium butyrate in the serum\|free medium and was entirely inhibited at 2 mmol/L sodium butyrate. The specific rate of glucose utilization was also reduced by 30% in the presence of sodium butyrate.In addition,EPO expression was not facilitated by the addition of sodium butyrate,but its addition may be helpful to maintain the stability of EPO productivity. |
| |
| Keywords: | sodium butyrate CHO cell recombinant human EPO cell culture |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
