| Rapid Amplification of 5‘ cDNA End of S.Liaotungensis Choline Monooxygenase Using Inverse PCR RACE |
| |
| 作者姓名： | 李秋莉 GaoXiaorong 等 |
| |
| 作者单位： | [1]BioengineeringDepartmentofDalianUniversityofTechnology,Dalian116012,P.R.China [2]BilogyDepartmentofLiaoningNormalUniversity,Dalian116029,P.R.China |
| |
| 摘 要： | Based on part of a known cDNA sequence of Suaeda Liaotungensis choline monooxygenase the author successfully cloned the 5‘ cDNA end of Suaeda Lianotungensis choline monooxygenase using Inverse PCR RACE with a specially designed 5‘-phosphated RT primer and two pairs of specific inverse PCR primers.Compared with the anchored PCR RACE,inverse PCR RACE has bettter specificity and higher amplification.
|
| 关 键 词： | DNA 放大 反演PCR RACE应用 |
| 本文献已被 维普 等数据库收录！ |
|
