| 用Blue一Sepharose·CL·6B亲和层析法分离纯化甲鱼和北京鸭LDH＿1、LDH＿5A亚基 |
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| 引用本文： | 王素云,陈谨,吴鹤龄. 用Blue一Sepharose·CL·6B亲和层析法分离纯化甲鱼和北京鸭LDH＿1、LDH＿5A亚基[J]. 北京大学学报(自然科学版), 1994, 0(4) |
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| 作者姓名： | 王素云 陈谨 吴鹤龄 |
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| 作者单位： | 北京大学生命科学学院 |
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| 摘 要： | 用ＢｌｕｅＳｅｐｈａｒｏｓｅ·ＣＬ．６Ｂ亲和层析方法分离纯化的甲鱼ＬＤＨ＿５、甲鱼ＬＤＨ＿１和北京鸭ＬＤＨ＿５，进行比较研究。从分子量、等电点、氨基酸组成、平均摩尔差异百分比及在ＢｌｕｅＳｅｐｈａｒｏｓｅ·ＣＬ·６Ｂ层析柱上洗脱特性几方面进行比较，结果表明，两个不同纲的动物（甲鱼和北京鸭）的ＬＤＨ＿５的Ａ亚基之间的差异大于同一种动物的ＬＤＨ＿５的Ａ亚基和Ｂ亚基之间的差异，并说明不同纲的动物ＬＤＨ＿５之间具有同源性。
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| 关 键 词： | 等电点；氨基酸组成；亲和层析 |
Using Blue Sepharose CL-6B System of Affinity Chromato-graphy to Isolate and Purify the A Subunit of Soft-shelled Turtle and Peking Duck |
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| WANG Suyun CHEN Jin WU Heling. Using Blue Sepharose CL-6B System of Affinity Chromato-graphy to Isolate and Purify the A Subunit of Soft-shelled Turtle and Peking Duck[J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 1994, 0(4) |
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| Authors: | WANG Suyun CHEN Jin WU Heling |
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| Abstract: | The result of comparing behavior of affinity chromatography,physical char-acteristics and amino acid composition and average mole persent difference of animals of 2 classes of vertibrates showed that the A subunit of soft-shelled turtle and Peking duck are mor homologous to each other than are the A and B subunit of soft-shelled turtle. |
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| Keywords: | isoelectric point aminoacid composition affinity chromatography |
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