Cholinesterases are down-expressed in human colorectal carcinoma |
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Authors: | M F Montenegro F Ruiz-Espejo F J Campoy E Muñoz-Delgado M Páez de la Cadena F J Rodríguez-Berrocal C J Vidal |
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Institution: | (1) Departamento de Bioquímica y Biología Molecular-A, Edificio de Veterinaria, Universidad de Murcia, Apdo. 4021, 30071 Murcia, Spain;(2) Departamento de Bioquímica, Genética e Inmunología, Facultad de Biología, Universidad de Vigo, 36200 Vigo, Spain;(3) Servicio de Análisis Clínicos, Hospital Virgen de la Arrixaca, 30120 Murcia, Spain |
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Abstract: | The aberrations of cholinesterase (ChE) genes and the variation of ChE activity in cancerous tissues prompted us to investigate
the expression of ChEs in colorectal carcinoma. The study of 55 paired specimens of healthy (HG) and cancerous gut (CG) showed
that acetylcholinesterase (AChE) activity fell by 32% and butyrylcholinesterase (BuChE) activity by 58% in CG. Abundant AChE-H,
fewer AChE-T, and even fewer AChE-R and BuChE mRNAs were observed in HG, and their content was greatly diminished in CG. The
high level of the AChE-H mRNA explains the abundance of AChE-H subunits in HG, which as glycosylphosphatidylinositol (GPI)-anchored
amphiphilic AChE dimers (G2A) and monomers (G1A) account for 69% of AChE activity. The identification of AChE-T and BuChE mRNAs justifies the occurrence in gut of A12, G4H and PRiMA-containing G4A AChE forms, besides G4H, G4A and G1H BuChE. The down-regulation of ChEs might contribute to gut carcinogenesis by increasing acetylcholine availability and overstimulating
muscarinic receptors.
Received 19 May 2006; received after revision 5 June 2006; accepted 5 July 2006 |
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Keywords: | Cancer cholinesterases glycosylphosphatidylinositol gut real-time PCR |
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