| 补骨脂胰蛋白酶抑制剂的亲和层析分离纯化和部分性质 |
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| 引用本文： | 朱苏闽,余萍.补骨脂胰蛋白酶抑制剂的亲和层析分离纯化和部分性质[J].福建师范大学学报(自然科学版),1990,6(3):68-72. |
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| 作者姓名： | 朱苏闽 余萍 |
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| 作者单位： | 福建师范大学高分子研究所，福建师范大学高分子研究所，福建师范大学高分子研究所，福建师范大学高分子研究所，福建师范大学高分子研究所，福建师范大学高分子研究所 |
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| 摘 要： | 本文介绍牛胰蛋白酶—对氨基苯砜乙基(ABSE)—Sepharose—4B的制备,及用此亲和吸附剂分离提纯补骨脂胰蛋白酶抑制剂的过程。用该方法对补骨脂胰蛋白酶抑制剂粗提物进行分离,可获得电泳单点纯样品。经SDS—PAGE电泳测定,该抑制剂的分子量为55000。等电聚焦电泳测定其等电点为pⅠ6.6,Chiff试剂染色为阴性。氨基酸组成分析結果表明含12种氨基酸,其中Gly、Glu含量最高。
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| 关 键 词： | 补骨脂 胰蛋白酶 抑制剂 亲和层析 |
Partly Characterization and Purification of Affinity Chromatography of Trypsin-inhibitor from Psoralea Corylifolia L. |
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| Zhu Sumin Yu Ping Huang Deqi Lin Shaoqin Yang Liangpeng Wu Xuchu.Partly Characterization and Purification of Affinity Chromatography of Trypsin-inhibitor from Psoralea Corylifolia L.[J].Journal of Fujian Teachers University(Natural Science),1990,6(3):68-72. |
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| Authors: | Zhu Sumin Yu Ping Huang Deqi Lin Shaoqin Yang Liangpeng Wu Xuchu |
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| Institution: | Institute of Polymer Science |
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| Abstract: | This article describes the preparation of trypsin-ABSE-Sepharose-4B and the purification of affinity chromatography of trypsin-inhibitor from Psoralea Corylifolia L. The trypsin-inhibitor electrophoreses as a single band on PAGE and its molecular weight is measured on SDS-PAGE to be 55000 daltor. Its band with pI points of 6.6 on polyaerylamide IEF is not stained by Chiff's agent. It shows by amino acid composition analysis the presence of 12 classes of amino acids and there are more Gly and Glu among them. |
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| Keywords: | trypsin-inhibitor psoralea corylifolia L affinity chromatography |
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