| 刺嫩芽中多酚氧化酶活性的影响因素研究 |
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| 引用本文： | 于爽,金建丽,王立凤,张学义.刺嫩芽中多酚氧化酶活性的影响因素研究[J].天津师范大学学报(自然科学版),2009,29(4):58-60. |
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| 作者姓名： | 于爽 金建丽 王立凤 张学义 |
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| 作者单位： | 牡丹江师范学院,生物系,黑龙江,牡丹江,157012 |
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| 基金项目： | 黑龙江省自然科学基金资助项目，黑龙江省教育厅科研项目 |
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| 摘 要： | 研究了pH、温度以及抑制剂与激活剂对从新鲜刺嫩芽中提取出的多酚氧化酶（PPO）活性的影响,及温度对其热稳定性的影响.结果表明：以邻苯二酚为底物,该酶的最适pH为4.4,在pH 4.0-4.8内有较高的稳定性;最适温度为12.5℃,在30℃以上失活;70℃热处理90 s可以钝化PPO的活性;抗坏血酸（Vc）,L-Cys对PPO有抑制作用,SDS对PPO有激活作用.
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| 关 键 词： | 刺嫩芽 多酚氧化酶 pH 温度 热稳定性 抑制剂 |
Influences of different factors on the enzyme activity of polyphenoloxidase in Aralia elata |
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| YU Shuang,JIN Jianli,WANG Lifeng,ZHANG Xueyi.Influences of different factors on the enzyme activity of polyphenoloxidase in Aralia elata[J].Journal of Tianjin Normal University(Natural Science Edition),2009,29(4):58-60. |
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| Authors: | YU Shuang JIN Jianli WANG Lifeng ZHANG Xueyi |
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| Institution: | (Department of Biology, Mudanjiang Normal University, Mudanjiang 157012, heilongjiang Province, China) |
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| Abstract: | The influences of pH, temperature, thermal stability, inhibitors and activators on the enzyme activity of polyphenoloxidase extracted from fresh Aralia elata are studied. With catechol as substrate, the optimum pH of the enzyme is 4.4, and the enzyme activity is higher with pH 4. 4--4.8. The optimum temperature is 12.5 ℃, and the temperature more than 30℃ results in the enzyme deactivation. Heat treating for 90 s at 70 ℃ passivates the enzyme activity. The enzyme activity could be restrained by ascorbate and L-Cys, but be activated by SDS. |
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| Keywords: | pH |
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