| D-核糖生产菌的紫外诱变表征及工艺 |
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| 引用本文： | 赵静波,吴兆亮,陈新征,荆迎军,刘虎.D-核糖生产菌的紫外诱变表征及工艺[J].天津大学学报(自然科学与工程技术版),2005,38(9):791-794. |
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| 作者姓名： | 赵静波 吴兆亮 陈新征 荆迎军 刘虎 |
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| 作者单位： | 河北工业大学化工学院,天津300130 |
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| 基金项目： | 河北省自然科学基金资助项目（201010）. |
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| 摘 要： | 确定合适的诱变剂量是D-核糖生产菌紫外诱变育种的一个重要原则，用正突变率作为评价指标无法确定最适诱变剂量，为此提出新的评价指标——平均正突变幅度，给出了其定义及计算方法，并将其应用于D-核糖生产菌的紫外诱变中，得到在15W紫外灯和距离25cm条件下不同照射时间的平均正突变幅度，并结合致死率曲线确定20s为最佳紫外照射时间。
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| 关 键 词： | D-核糖 紫外诱变 诱变剂量 平均正突变幅度 |
| 文章编号： | 0493-2137(2005)09-0791-04 |
| 收稿时间： | 09 22 2003 12:00AM |
| 修稿时间： | 11 18 2003 12:00AM |
Representation and Technical Process of Ultraviolet Induced Mutation of Strain for Producing D-Ribose |
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| Zhao JingBo;Wu ZhaoLiang;Chen XinZheng;Jing YingJun;Liu Hu.Representation and Technical Process of Ultraviolet Induced Mutation of Strain for Producing D-Ribose[J].Journal of Tianjin University(Science and Technology),2005,38(9):791-794. |
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| Authors: | Zhao JingBo;Wu ZhaoLiang;Chen XinZheng;Jing YingJun;Liu Hu |
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| Abstract: | The use of appropriate mutagen dosage is important in ultraviolet (UV) induced mutation of D-ri-bose producing strain. But it was hard to find the optimal mutagen dosage when positive mutation rate was used as the evaluating index. So a new evaluating index-average positive mutation extent was proposed and its definition and calculating method were given. When it was used in UV induced mutation of D-ribose producing strain under the condition of 25 cm below a 15 W UV lamp, the value of average positive mutation extent of different irradiation intervals was got. Combining the average positive mutation extent with lethal rate curve, 20 s was found as the best irradiation time. |
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| Keywords: | D-ribose ultraviolet induced mutation mutagen dosage average positive mutation extent |
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