GnRH-A抗原主动免疫雄兔对血液Fe、Cu、Zn的影响

目的:探讨促性腺激素释放激素类似物-A(GnRH-A)主动免疫时动物血液微量元素的变化规律,为深入研究GnRH免疫去势的作用机理提供科学依据.方法:用自制的GnRH-A抗原乳剂1.0 mL对兔子进行皮下注射,美国热电SOLAAR S4型原子吸收光谱仪测定Fe、Cu、Zn.结果:Zn的含量在第4周明显高于免疫去势前的水平(P<0.05),Cu和Fe与免疫去势前无显著差异(P>0.05).结论:GnRH-A主动免疫对雄兔血液微量元素无明显影响.

Effects of GnRH-A Active Immunocastration on Blood Fe, Cu and Zn Contents in Rabbits

Objective: To explore the changes of blood microelements contents of(iron,cupper and zinc) in male rabbits when they are immunized actively by injecting gonadotropin releasing hormone analogues(GnRH-A),and to provide researchers some scientific basis for study of the effects of GnRH immunization castration and mechanism profoundly in animals.Methods: 1.0 mL GnRH-A antigen emulsion were subcutaneously injected into experimental rabbits,contents of iron,cupper and zinc were measured with SOLAAR S4 atomic absorption spectrometer.Results: Zinc contents in the first 4 weeks was significantly higher than the level before the immune castration(P<0.05),Cupper and iron contents had no-significantly difference compared with the level before immunocastration(P>0.05).Conclusion: GnRH-A active immunization for male rabbit had no remarkable effects on microelements in male rabbit.