小鼠腹腔注射卡介苗加脂多糖建立急性免疫性肝损伤的实验研究

目的比较卡介苗(BCG)加脂多糖(LPS)小鼠腹腔注射和尾静脉注射建立急性免疫性肝损伤模型的优劣。方法用不同剂量BCG和LPS腹腔注射诱导昆明小鼠建立急性免疫性肝损伤模型,与BCG 0.5 mg/只和LPS 7.5μg/只联合尾静脉注射诱导昆明小鼠建立急性免疫性肝损伤模型比较;以小鼠血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)水平变化及肝脏病理学检查指标作为肝损伤判断标准。结果BCG+LPS小鼠腹腔注射所造急性免疫性肝损伤模型,ALT和AST随注射剂量增加均逐步增高;第6组(BCG 0.5 mg/只+LPS7.5μg/只)和第13组(BCG1 mg/只+LPS15μg/只)相对较高。与空白组比较,ALT升高约3倍,AST升高1~3倍;肝组织病理损伤则随着BCG和LPS剂量增加逐渐升高。第5组大部分小鼠出现Ⅰ级或Ⅰ级以上肝细胞损害;第13组大部分小鼠出现Ⅱ级或Ⅱ级以上肝细胞损害。但与尾静脉注射造模相比,小鼠腹腔注射造模肝细胞损伤的特异性实验室指标ALT和AST表达不高,肝组织病理损伤较轻。结论"BCG+LPS"小鼠尾静脉注射建立急性免疫性肝损伤模型优于腹腔注射建立免疫性肝损伤模型。

An Experimental Study of Acute Immune Liver Injury Model Established by Intraperitoneal Injection of BCG and LPS on Mouse

Objective To compare with the merits and demerits of intraperitoneal injection in mouse and vein injection in mouse to establish acute immune liver damage tail model by Bacillus Calmette-Guerin(BCG) and lipopolysaccharide(LPS).Methods To compare with different doses BCG of and different doses of LPS induced by intraperitoneal injection of immune mouse established acute liver injury model,and BCG 0.5 mg/only and LPS 7.5 μg/only combined tail vein injection induced Kunming mouse to established the mouse model of acute immune liver injury.To serum transaminase levels in mice and liver pathology as index of liver injury inspection criteria.Results "BCG+LPS" mice by intraperitoneal injection to built the acute immune liver damage model,ALT and AST are increased with the dose gradually increased in section:No.5 Group(BCG 0.5 mg/only+LPS 7.5 μg/only) and No.13 Group(BCG 1 mg/only+LPS 15 μg/ only) is relatively higher.Compared with the control group,ALT increased by about three times,AST is also 1～3 times higher basic;Both of them pathological liver tissue injury appeared with gradually increased doses of BCG and LPS. No.5 Group:most mice Ⅰ appeared Ⅰgrade or above grade Ⅰ liver cell damage;13 Group appeared Ⅱ level in most mice or above grade Ⅱ liver cell damage.But to compared with the tail vein injection modeling,modeling mice by intraperitoneal injection of liver cell injury expression of specific laboratory index of ALT and AST is not high,and liver pathology injury is also lighter.Conclusion Mouse tail vein injection of BCG and LPS established Acuat immune liver damage model is superior to the establishment of intraperitoneal injection of immune liver damage model.