金纳米粒子修饰有机聚合物整体柱的制备及其在柱SERS检测研究

以甲基丙烯酸丁酯(BMA)为单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,以正丙醇和1,4-丁二醇为二元致孔剂,在毛细管内由热引发进行原位聚合制备聚甲基丙烯酸酯毛细管整体柱.通过物理吸附作用将不同粒径的金纳米粒子(AuNPs)修饰在整体柱材料孔表面并用于表面增强拉曼散射(SERS)光谱分析.利用透射电子显微镜(TEM)、紫外-可见吸收光谱(UV-Vis)、扫描电子显微镜(SEM)对AuNPs形貌、吸收光谱及吸附AuNPs前后整体柱的形貌进行表征.以对巯基苯胺(PATP)为探针分子,采用波长为633nm的激发光作为激发光源,研究不同粒径AuNPs修饰的聚甲基丙烯酸酯整体柱的在柱表面增强拉曼光谱(SERS)性能.结果表明,该整体柱SERS基底具有良好的SERS增强效应.随着AuNPs粒径的增大,基底的SERS活性逐渐增强.

Preparation of porous polymer monolith modified with gold nanoparticles and on-colunm surface-enhanced Raman spectroscopy detection

A porous polymethacrylate ester-based capillary monolithic column was prepared using in situ ccr polymerizing butylmethacrylate （BMA） and ethylenedimethacrylate （EDMA） in binary porogenic solvent 1,4 butanediol and 1-propanol. After injecting a gold nanoparticles （AuNPs） colloid into the monolith, a homogeneous coverage of AuNPs was physically adsorbed on the monolith surface. Transmission electron microscopy （TEM） and UV-Vis spectrometer were used to collect the TEM images and the extinction spectra of the nanoparticles colloid, respectively. Scanning electron microscope （SEM） was utilized to record the morphology of the organic monolith. We used p-aminothiophenol （PATP） as a probing molecule, and the SERS effect was investigated on AuNPs-modified BMA monolith under the excitation line of 633 nm. It was concluded that AuNPs--modified BMA monolith showed obvious SERS enhancement, and the SERS activity was dependent on the size of AuNPs.