| 大枣组织培养中外植体预处理的研究 |
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| 引用本文： | 陈宗礼,延志莲.大枣组织培养中外植体预处理的研究[J].延安大学学报(自然科学版),1994,13(1):66-69. |
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| 作者姓名： | 陈宗礼 延志莲 |
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| 摘 要： | 本文给出了大枣组织培养中外植体预处理的优化方法。灭菌程序是：外植体→修剪→洗衣粉水洗净→蒸馏水冲洗滤干→７５％乙醇１０－１２利→饱和漂白粉上清波加２滴吐温－３０的混合液２０－２５钟分→无菌水冲洗４－５次→切段接种。保存方法是；外值体经蒸馏水冲净后插入蒸馏水（或保鲜液）中，于４±１℃的条件下保存，在５天内接种。这些方法对植物组织培养有普遍的参考意义。文中还讨论了外植体低温保存的有关机理。
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| 关 键 词： | 大枣外植体，灭菌，保存 |
STUDIES OF THE EXPLANT PRETREATMENT IN TISSUE CULTURE OF ZIZYPHUS JUJUBA MILL |
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| Chen Zongli,Yan Zhilan,Qi Long.STUDIES OF THE EXPLANT PRETREATMENT IN TISSUE CULTURE OF ZIZYPHUS JUJUBA MILL[J].Journal of Yan'an University(Natural Science Edition),1994,13(1):66-69. |
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| Authors: | Chen Zongli Yan Zhilan Qi Long |
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| Institution: | Department of BioLogy |
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| Abstract: | The paper gives the good method of pretreatment of the explant in tissue culture of zizyphus jujuba mill, the disinfectant procedure and the method of keeping explants as well. |
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| Keywords: | The explants of zizyphus jujuba mill disinfect preservation |
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