金盏菊叶片组织培养再生植株

金盏菊离体叶片培养采用MS培养基,诱导愈伤组织时,附加2,4—D0.5mg/l、NAA0.5—1mg/l、6—BA2mg/l、水解乳蛋白500mg/l。诱导愈伤组织分化时则附加6—BA2mg/l、NAA0.5mg/l。诱导根生成时,附加IBA2mg/l。所有培养基的蔗糖浓度均为3％,PH5.8—6.0。经一段时间培养后获得金盏菊再生植株。

THE TISSUE CULTURE OF LEAVES AND REGENERATION OF PLANTLETS OF CALENDULA OFFICINAL1S

Leaf explants of calendula officinalis were cultured on the MS media Supplimented with 2,4—D 0.5mg/l、NAA 0.5—1mg/l, 6—BA2mg/l、LH 500mg/l for the induction of Callus formation, or Supplimented with 6—BA2mg/l、NAA0.5mg/l for the induction and callus differentiation when roots were especially induced, only IBA 2mg/l was Supplimented: All(meolia) above were added with Sucrose with Concentration of 3％, After a period of culture regeneration of plantlets was obtained。