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Harvesting large amounts of Drosophila embryos at precisely defined stages
Authors:R Messeguer  J Piñol  O Cabré
Institution:(1) Departamento de Genética, Facultad de Ciencias, Universidad Autónoma de Barcelona, Bellaterra, Barcelona, Spain
Abstract:Summary A simple method is described which makes it possible to eliminate the retained eggs ofDrosophila melanogaster and to harvest a large amount of embryos at a defined stage of development. It is based upon carbon dioxide anesthesia, which brings about the expulsion of eggs remaining in the uterus.
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