Folding patterns of chromatin in chicken erythrocyte studied by using AFM and TEM |
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| Institution: | LIU Zhenxia\+1, ZHOU Meiyun\+1, XIE Hengyue\+1, QIAN Ruolan\+1\+*, ZHANG Yi\+2, HU Jun\+2 and LI Minqian\+2 1. Shanghai Institute of Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China; 2. Shanghai Institute of Nuclear Research, Chinese Academy of Sciences, Shanghai 201800, China. * Corresponding author. |
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| Abstract: | Four folding patterns (～15, ～30-40, ～60 and ～90-110 nm) of the higher_order structure of chromatin in chicken erythrocytes have been observed with an atomic force microscope (AFM) under near natural conditions. Results showed that the chromatin in the interphase nucleus of chicken erythrocyte was not in a disorganized form but represented a higher_order conformation. The thin sections of the chromatin in the nucleus of chicken erythrocyte were examined with a transmission electron microscope (TEM). Compared with TEM, AFM had the advantages that the samples did not need the preparation procedures such as fixing, sectioning and staining, and thus were closer to their native condition. |
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| Keywords: | chromatin structure nucleus of chicken erythrocyte AFM TEM |
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