| 甜味蛋白thaumatin-PDI二价植物表达载体的构建 |
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| 引用本文： | 杨奇志,陈少敏,赵琦.甜味蛋白thaumatin-PDI二价植物表达载体的构建[J].首都师范大学学报(自然科学版),2005,26(2):59-62,68. |
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| 作者姓名： | 杨奇志 陈少敏 赵琦 |
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| 作者单位： | 首都师范大学生物系,北京,100037;首都师范大学生物系,北京,100037;首都师范大学生物系,北京,100037 |
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| 基金项目： | 国家自然科学基金资助 (A3 0 0 70 488) |
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| 摘 要： | 将thaumatin cDNA和PDI基因的上游分别连上启动子，下游连上终止子，通过基因重组技术，克隆到植物表达载体pCAMBIAl302中，构建成一个同时含有thaumatin基因和PD／基因的新的植物二价表达载体pCAMBIAl302-tha-PDI.经测序，该表达载体中的thaumatin基因与PDI基因的编码阅读框序列完全正确。
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| 关 键 词： | thaumatin PDI 表达载体 |
Construction of the Plant Expressing Vector Containing thaumatin Gene and PDI Gene |
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| Yang Qizhi,Chen Shaomin,Zhao Qi.Construction of the Plant Expressing Vector Containing thaumatin Gene and PDI Gene[J].Journal of Capital Normal University(Natural Science Edition),2005,26(2):59-62,68. |
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| Authors: | Yang Qizhi Chen Shaomin Zhao Qi |
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| Abstract: | The promoter was connected to the upriver sections of the gent PDI and the thaumatin cDNA,the terminator was also connected to their downriver sections.With the base of the plant expressing vector pCAMBIA1302,we constructed a new plant expressing vector pCAMBIA 1302-tha-PDI,which contained the gene thaumatin and the gene PDI.After sequencing,the base sequences of the gene thaumatin and the gene PDI in pCAMBIA 1302-tha-PDI were exactly right. |
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| Keywords: | thaumatin PDI expressing vector |
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