Mass fragmentographic determination of cholecalciferol and 25-hydroxycholecalciferol in human serum |
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| Authors: | B Zagalak H -Ch Curtius Rosmarie Foschi Gabrielle Wipf U Redweik Maria-Jolanta Zagalak |
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| Institution: | (1) Department of Pediatrics, Division of Medical Chemistry, University of Zurich, Kinderspital, CH-8032 Zurich, (Switzerland) |
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| Abstract: | Summary The serum extracts were purified by column-, thin layer- and high pressure liquid chromatography. Deuterated cholecalciferol and deuterated 25-hydroxycholecalciferol were used as internal standards. The quantitative analysis was performed using GC-mass fragmentography technique of TMS-ethers. |
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