预处理玉米秸秆中抑制物对酶解与发酵影响研究

玉米秸秆在预处理过程中会生成酵母抑制物，不利于发酵产乙醇。本文考察了七种模型抑制物对酵母菌发酵乙醇的影响，并对预处理玉米秸秆（PCS）进行了脱毒试验，研究了酶解与同步糖化发酵过程。得到，对酿酒酵母FE-B抑制作用由强到弱为苯系化合物＞呋喃衍生物＞弱酸类；水洗至中性后挤压PCS的酶解和发酵效果均好于未处理和挤压处理PCS，在固含量为30%的酶解与发酵体系中，72 h葡萄糖浓度为12.23%，发酵24 h乙醇浓度达到5.82 w%。

Effect of Inhibitors in Pretreated Corn Stalk on Enzymatic Hydrolysis and Microbial Fermentation

Inhibitors are produced during pretreatment of corn stalk, which is limited to ethanol product. The effect of seven model inhibitors on ethanol production using cerevisiae was researched and detoxification experiment of pretreated corn stalk (PCS) was conducted. Enzymatic hydrolysis and Saccharification process were also studied in this article. Benzene hydrocarbons inhibit saccharomyces cerevisiae more seriously than furan derivatives and weak acids respectively. The enzymatic hydrolysis and fermentation results of PCS under extrusion treatment after cleaning with water till to neutral were better than untreated and singly extrusion. Glucose and ethanol concentration reached 12.23 %(72h) and 5.82 w%(96h), respectively, in 30% total solids content system.