| Transformation of Streptococcus zooepidemicus with Genes Responsible for Polyhydroxybutyrate Synthesis |
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| 作者姓名： | 吴小明 高海军 田格 陈国强 |
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| 作者单位： | WU Xiaoming，GAO Haijun，TIAN Ge CHEN Guoqiang Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China |
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| 摘 要： | IntroductionGenetic manipulation of bacteria has become apowerful tool for elucidating fundamentalbiological mechanisms.While many techniques areavailable to introduce foreign DNA into severalbacterial species1 ] ,some bacteria,such asStreptococcus spp.,have proven to be resistant totransformation with mostprotocols thathave beendescribed.In addition,there are large differencesin the transform ability among various strains ofStreptococcusspp. Streptococci have been divided into six majorph…
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Transformation of Streptococcus zooepidemicus with Genes Responsible for Polyhydroxybutyrate Synthesis |
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| WU Xiaoming,GAO Haijun,TIAN Ge CHEN Guoqiang.Transformation of Streptococcus zooepidemicus with Genes Responsible for Polyhydroxybutyrate Synthesis[J].Tsinghua Science and Technology,2002,7(4). |
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| Authors: | WU Xiaoming GAO Haijun TIAN Ge CHEN Guoqiang |
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| Institution: | WU Xiaoming,GAO Haijun,TIAN Ge CHEN Guoqiang Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing 100084,China |
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| Abstract: | A procedure for transformation of intact Streptococcus zooepidemicus cells by electroporation was developed through a systematic examination of the effects of various parameters, including growth conditions, electric field strengths used for electroporation, and concentrations of plasmid used for transformation. Efficiencies higher than 104 cfu/μg(cfu, clone forming unit) plasmid DNA were obtained for Streptococcus zooepidemicus H2004 cells. Results demonstrate that the broad-host-range plasmid pDL276 can be replicated in Streptococcus zooepidemicus H2004 and foreign genes responsible for polyhydroxybutyrate (PHB) synthesis inserted into the pDL276 can be successfully expressed in the transformant, in which PHB is detected using the Fourier transform-infrared spectroscopy (FT-IR) method. |
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| Keywords: | electroporation Streptococcus polyhydroxybutyrate(PHB) Fourier transform infrared spectroscopy (FT IR) |
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