| New Equation of State for Osmotic Pressures in Aqueous Saline Solutions of Lysozyme |
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| 作者姓名： | 林阳政 李以圭 陆九芳 |
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| 作者单位： | LIN Yangzheng，LI Yigui *，LU Jiufang Department of Chemical Engineering，Tsinghua University，Beijing 100084，China |
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| 基金项目： | the National Natural Science Foundationof China(No. 2 9736 170 ) |
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| 摘 要： | IntroductionIn the biotechnology industry,the separation andpurification of proteins from aqueous solutions isan increasingly important process. A goodunderstanding of the non- idealities and phasebehavior in aqueous electrolyte solutions ishelpful. Moon et al.1] measured a series ofexperimental osmotic pressure data for hen- egg-write lysozyme concentrations from about4 2 2g/L in aqueous ( NH4) 2 SO4,( NH4) 2 C2 O4and( NH4) 2 HPO4solutions at ionic strength of1 3.5mol/kg and p H 4,7or …
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New Equation of State for Osmotic Pressures in Aqueous Saline Solutions of Lysozyme |
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| Abstract: | A new equation of state is proposed to correlate the osmotic pressure data for aqueous lysozyme solutions with (NH4)2SO4, (NH4)2C2O4 and (NH4)2HPO4 at ionic strengths of 13.5 mol/kg and pH 4, 7 or 8 with only one adjustable parameter instead of the classical Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. The Carnahan-Starling equation represents the contribution of the hard sphere repulsion to the osmotic pressure. The attractive dispersion interaction is represented by the Lennard-Jones potential expressed by the equation of Cotterman et al. based on perturbation theory. The double-layer repulsion interaction is represented by Yukawa potential expressed by the equation of state of Duh and Mier-Y-Teran based on mean spherical approximation. The total average relative deviation of the correlation of the osmotic pressure data is 1.68%. |
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| Keywords: | osmotic pressure lysozyme Yukawa potential Lennard Jones potential equation of state |
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