| Heat Denaturation of Protein Structures and Chlorophyll States in PSII Membranes |
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| 引用本文： | 李冬海,阮翔,许强,王可玢,公衍道,匡廷云,赵南明. Heat Denaturation of Protein Structures and Chlorophyll States in PSII Membranes[J]. 清华大学学报, 2002, 7(4) |
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| 作者姓名： | 李冬海 阮翔 许强 王可玢 公衍道 匡廷云 赵南明 |
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| 作者单位： | LI Donghai，RUAN Xiang，XU Qiang，WANG KebinGONG Yandao，KUANG Tingyun，ZHAO Nanming State Key Laboratory of Biomembrane and Membrane Biotechnology，Department of Biological Sciences and Biotechnology，Tsinghua University，Beijing 100084，China; P |
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| 基金项目： | Supported by the State Key Basic Research and Development Plan (No.G19980 10 10 0 )，the National Natural Science Foundation of China(No.3 9890 3 90 ) |
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| 摘 要： | IntroductionPhotosystem II (PSII) is a large supramolecularpigment-protein complex found in the thylakoidmembranes of green plants,algae andcyanobacteria.Its main role is to drive light-induced electron transfer from water toplastoquinone with a concomitant production ofmolecular oxygen.PSII membranes consist of anouter antenna portion of light-harvestingchlorophyll (Chl) a/b binding complexes (LHCII)and a core fraction.The core fraction is composedof an inner antenna of membrane-bound …
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Heat Denaturation of Protein Structures and Chlorophyll States in PSII Membranes |
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| Abstract: | Heat denaturation is an important technique in the study of the structure and function of photosynthetic proteins. Heat denaturation of photosystem II (PSII) membrane was studied using circular dichroism (CD) spectroscopy, differential scanning calorimetry (DSC) and oxygen electrode. Complete loss of oxygen-evolving activity of the PSII membrane was observed at temperatures below 45℃. The decrease of excitonic interaction between chlorophyll molecules occurred more rapidly than the change of the protein secondary structure of the PSII membrane at temperatures above 45℃. The results indicate that the protein secondary structure of the membrane proteins in PSII membranes is more stable than the excitonic interaction between chlorophyll molecules during heat denaturation. |
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| Keywords: | photosystem II (PSII) oxygen evolving activity circular dichroism (CD) differential scanning calorimetry (DSC) excitonic interaction heat denaturation |
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