苯甘氨酸肽固定相制备及其色谱性能评价

利用＂点击化学＂（Click Chemistry）合成了一系列L-苯甘氨酸为母体结构的疏水固定相（L-苯甘氨酸甲酯、L-苯甘氨酸二肽、L-苯甘氨酸三肽）;合成过程中,一个结构稳定的重氮转移试剂——磺酰叠氮咪唑被用于在温和条件下将甘氨酸中的氨基转化为叠氮基的试剂。初步的色谱评价表明,随着氨基酸单元的增加,其对模型化合物的...

Synthesis and Chromatographic Performance of Click Phenylglycine Based Peptide Stationary Phases

A series of phenylglycine based hydrophobic stationary phases were synthesized by click chemistry,such as L-methyl phenylglycine,L-phenylglycine dipeptide and L-phenylglycine tripeptide.In these processes,a shelf-stable diazotransfer reagent(imidazole-1-sulfonyl azide hydrochloride) was utilized to transfer the amino group in phenylglycine on mild conditions.Primary chromatographic evaluation showed that the retention time of the model solutes increased with the increase of the phenylglycine unit.Elemental ...