| 黄孢原毛平革菌低温产酶条件优化及酶学性质研究 |
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| 引用本文： | 孟庆辉,代莉蓉,崔艳红.黄孢原毛平革菌低温产酶条件优化及酶学性质研究[J].西北民族学院学报,2009,30(2):66-70. |
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| 作者姓名： | 孟庆辉 代莉蓉 崔艳红 |
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| 作者单位： | 孟庆辉(西北民族大学,生命科学与工程学院,甘肃,兰州,730030);代莉蓉(农业部沼气科学研究所,四川,成都,610041);崔艳红(河南科技学院动,物科学学院,河南,新乡,453003) |
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| 基金项目： | 西北民族大学研究生科研创新项目 |
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| 摘 要： | 以酶活力为评价指标,在10℃对黄孢原毛平革菌产酶条件进行优化,并对其部分酶作用特性进行了研究.结果:该菌在10℃下产LiP、MnP和Lac的最佳碳源是葡萄糖,最佳氮源是牛肉膏,最佳培养时间是72~108 h.LiP、MnP和Lac的最适反应pH范围均为4.4~4.8,最适底物浓度是0.8~1.2 mmol/L;金属离子Cu2+,Ca2+对LiP、MnP和Lac有激活作用.Fe2+对三种酶活有一定的抑制作用;而Na+则完全抑制LiP的活性,Zn2+、Mg2+对三种酶活影响不大.
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| 关 键 词： | 黄孢原毛平革菌 木质素酶 锰过氧化物酶 漆酶 统计分析 |
Research on Phanerochaete Chrysosprium in Low Temperature Conditions Product Enzymes and Identity Study |
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| Institution: | MENG Qing - hui, DAI Ling - rong, CUI Yan - hong (1. Life Science and Engineering College of Northwest University for Nationalities, Lanzhou Gansu 730030, China;2. Biogas Institute of the Ministry of Agriculture, Chengdu, Sichuan, 610041, China;3. Animal Science College, Henan Institute of Science and Technology, XinXiang, Henan, 453003, China) |
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| Abstract: | The study enzyme vitality indicators for the evaluation through off enzyme optimization in the 10℃ conditions, and their role identity of enzymes studied. Results： Phanerochaete ehrysosprium off ligninase, manganese peroxidase and Laecase conditions is the best; the best net is glucose （net） ; Beef is the best source of nitrogen ointment; The best time is 72 - 108 hours of training. Ligninase, manganese peroxidase and Laeease are the optimal pH range 4.4 - 4.8, optimal concentrations of chemicals is 0.8 - 1. 2retool/L, Metal ions Cu2 ＋, Ca2 ＋ to ligninase, manganese peroxidase and Laecase have brought along role, Fe2 ＋ vitality of the three enzymes have certain effect, and Na ＋ the active enzyme is completely curb painting, Zn2 ＋ ,Mg2 ＋ little impact on the vitality of the three enzymes. |
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| Keywords: | Phanerochaete chrysosprium ligninase manganese peroxidase Laccase statistical analysis |
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