| 花药培养过程中的灭菌与消毒 |
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| 引用本文： | 王裔娜.花药培养过程中的灭菌与消毒[J].河南教育学院学报(自然科学版),2011(3):47-50. |
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| 作者姓名： | 王裔娜 |
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| 作者单位： | 河南农业大学园艺学院; |
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| 摘 要： | 利用植物组织培养技术对花药进行组织培养,结合实验操作经验,总结了培养基、无菌室和超净工作台的灭菌方法,论述了工具器皿的消毒过程,分析了花药的预处理与消毒方法,给出了常用消毒剂的使用方法.
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| 关 键 词： | 花药 培养 灭菌 消毒 温度 |
Disinfection and Sterilization in Anther Cultivation Progress |
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| WANG Yi-na.Disinfection and Sterilization in Anther Cultivation Progress[J].Journal of Henan Education Institute(Natural Science Edition),2011(3):47-50. |
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| Authors: | WANG Yi-na |
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| Institution: | WANG Yi-na(Institute of Horticulture,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | By application of plant tissue cultivation technology on cultivation of anther tissue,and combining operation experience,summarizes sterilization method of media,sterile room and ultra clean bench.The sterilization process of vessels and tools is discussed.Furthermore,analyzes method of anther pretreatment and its disinfection,and shows usage of common disinfectants. |
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| Keywords: | anther cultivation sterilization disinfection temperature |
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