甲型流感病毒PB1-PA蛋白作用位点的发现与分析

 基于共进化理论，探究了甲型流感病毒PB1蛋白与PA蛋白上具有共同进化可能性的保守九聚片段 (C9MP)。结构信息显示PB1蛋白的第1-15位氨基酸与PA蛋白的第239-716位氨基酸具有相互作用域；对该区域变异分布的分析发现，PA蛋白第670位氨基酸Q所在的C9MP与PB1蛋白的第9位氨基酸F、第12位氨基酸V和第13位氨基酸P所在的C9MP在PB1-MP1相互作用面上具有最低的共进化值。结合DSSP程序的分析表明，由PA蛋白第670位氨基酸Q与PB1蛋白的第9位氨基酸F、第12位氨基酸V与第13位氨基酸P构成的区域可能成为潜在的相互作用位点。

Discovery and Analysis of Protein-Protein Interaction Sites of PB1-PA Nuclease Complex of Influenza A Virus

Based on co-evolution theory, this research tries to locate the protein interaction sites in conserved 9 mer peptides (C9MP) between PB1 protein and PA protein in the nuclease complex of influenza A virus. There exist interaction regions between sites 1-15 amino acids of PB1 protein and sites 239-716 amino acids of PA protein from structure message. It is revealed by variation analysis to this region that, on PB1-MP1 contact surface, there is a minimum co-evolution value between the conserved 9 mer peptide where Q670 of PA protein is located and the conserved 9 mer peptide where F9, V12 and P13 of PB1 protein are located. Combined with DSSP analysis results, the regions formed by four amino acids, Q670 of PA protein, F9, V12 and P13 of PB1 protein, are potential interaction sites.