| 佛手饮料废渣中分离柠檬苦素的研究 |
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| 引用本文： | 许杭琳,邓刚,焦聪聪,孔黎春,张艺馨,黄群晓.佛手饮料废渣中分离柠檬苦素的研究[J].浙江师范大学学报(自然科学版),2012(3):322-325. |
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| 作者姓名： | 许杭琳 邓刚 焦聪聪 孔黎春 张艺馨 黄群晓 |
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| 作者单位： | 浙江师范大学化学与生命科学学院 |
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| 基金项目： | 国家自然科学基金资助项目(21006098);浙江师范大学创新科研项目(2010-2013) |
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| 摘 要： | 采用Sephadex LH-20凝胶柱色谱分离纯化了佛手饮料废渣中的柠檬苦素，分离条件：检测器波长为207nm；柱体积为112．5mL，分离柱内径为1．5cm；流动相为甲醇，流速为0．5mL／min．用核磁共振法对分离产物进行了结构鉴定．通过高效液相色谱法测定了佛手饮料废渣中柠檬苦素的含量，测定条件：采用美国AGT Venusil XBP（L）ODSC18柱（250mm×4．6mm，10μm）；流动相y（乙腈）：y（水）=45：55，流速为0．8mlMmin；柱温为25℃；检测波长为207nm．结果显示，佛手饮料废渣中柠檬苦素含量为1．73mg／g，相对标准偏差为1．1％．
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| 关 键 词： | 佛手 废渣 柠檬苦素 葡聚糖凝胶LH-20 高效液相色谱 |
Extraction of limonin from residue of berganot juice |
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| XU Hanglin,DENG Gang,JIAO Congcong,KONG Lichun,ZHANG Yixin,HUANG Qunxiao.Extraction of limonin from residue of berganot juice[J].Journal of Zhejiang Normal University Natural Sciences,2012(3):322-325. |
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| Authors: | XU Hanglin DENG Gang JIAO Congcong KONG Lichun ZHANG Yixin HUANG Qunxiao |
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| Institution: | (College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China) |
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| Abstract: | Limonin in waste residue of bergamot was separated by Sephadex LH-20 column chromatography,and the wavelength of detector was set to 207 nm;column volume was 112.5 mL,the diameter of column was 1.5 cm;with methanol as 100% mobile phase and flow rate flow rate was 0.5 mL/min,NMR was used to structural identification after the separation.HPLC was used to determine the quantity of limonin.Chromatographic conditions: American AGT Venusil XBP(L) ODS C18(250 mm×4.6 mm,10 μm) column with acetonitrile-water,45 ∶ 55(V/V) as mobile phase and flow rate 0.8 mL/min.The column temperature was controlled at 25 ℃ and the wavelength of detector was set to 207 nm.The results showed that the RSD was 1.1% and the limonin content in waste residue of bergamot under those conditions reached 1.73 mg/g. |
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| Keywords: | fingered citron waste residue of bergamot limonin Sephadex LH-20 HPLC |
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