镉胁迫下萝卜根系蛋白质组的差异表达

分别利用浓度为10、50、100、150μmol／L的CdCl2处理萝卜幼苗,分析根系全蛋白质组和根系线粒体蛋白质组发生的变化,并对其中100μmol／L的CdCl2诱导下萝卜根系的差异表达蛋白质组作详细分析,利用质谱技术鉴别差异蛋白的归属．根系全蛋白中有22个蛋白质斑点显示出差异表达,其中11个斑点（斑点4、斑点9～18）下调,4个癍点（宽点19～22）上调,7个斑点（斑点1～3,5-8）被抑制表达．线粒体中有22个蛋白质斑点显示差异表达,其中19个癍点（斑点l～19）下调,2个斑点（斑点20～21）上调,斑点22被诱导合成．经CdCl2诱导后,萝卜根系内的蛋白质组产生明显的差异表达现象,这些蛋白质分别是：cruciferin,maturase K,DNA—bindingprotein,defence-related protein, F-box family protein, F-actin capping protein beta subunit, transcription factor, monodehydroascorbate reductase, chaperone binding protein, SHL1, RNA polymerase beta chain, ATF3, putative acyl transferase 4, transposon protein putative mutator sub-class, transposon protein putative En/spm sub-class, retransposon protein, putative Tyl-copia sub-class, isocitrate lyase, phytochrome kinase substrate-related, phosphoenolpyruvate carboxylase, serine/threonine-protein kinase, ATP synthase beta chain, ARol-like protein 4, catalase isozyme 1, pentatricopeptide （PPR）repeat-countaining protein, cytochrome p450, DNA polymerase I , trihelix transcription factor, NADH dehydrogenase submit F, cycline-dependent kinase, F-box protein-related, ATP synthase subunit beta, mitochondrial precursor, glucan phosphorylase putative, zinc finger family protein, heat shock protein 26, peroxiredoxin type2 putative, alcoholdehydrogenase．这些差异表达的蛋白质,其功能涉及DNA、RNA及蛋白质合成,基因转录、转座与内含子剪接,细胞信号转导与结构维持,以及不良环境条件下的防御与能量代谢等方面．

Differential expression of protemics in radish roots under cadmium stress

The Raphanus sativus seedlings were treated with 10, 50, 100 and 150 μmol/L CdCl2 to analyze the changes of root whole proteome and root mitochondrial proteins. The differential expression of protein in the radish root treated with 100 μmol/L CdCI： was studied especially. Mass-spectrometric technique was employed to identify the differential proteins. In root total proteins, 22 protein spots were altered in protein abundance when compared with control. In 22 identified proteins, 11 protein spots （spot 4, spot 9-18） were down-regulated in abundance and 4 protein spots （spot 19-22） were up-regulated in abundance, The expression of 7 protein spots （spot 1-3, spot 5-8） was inhibited. In mitochondrial pro- teins, 22 protein spots showed change in protein abundance. Among these spots, 19 protein spots （spot 1-19） were down- regulated in abundance and 2 protein spots （spot 20-21 ） were up-regulated in abundance, and a new protein spot, spot 22, was induced. Induced by CdC12, the root proteome showed obvious change in abundance. These identified Cd^2＋ responsive proteins were： cruciferin, maturase K, DNA-binding protein, defence-related protein, F-box family protein, F-actin capping protein beta subunit, transcription factor, monode hydroascorbate reductase, chaperone binding protein, SHL1, RNA polymerase beta chain, ATF3, putative acyl transferase 4, transposon protein putative mutator sub-class, transposon protein putative En/spm sub-class, retransposon protein, putative Tyl-copia sub-class, isocitrate lyase, phy- tochrome kinase substrate-related, phosphoenolpyruvate carboxylase, serine/threonine-protein kinase, ATP synthase beta chain, ARol-like protein 4, catalase isozyme 1, pentatricopeptide （PPR） repeat-countaining protein, cytochrome p450, DNA polymerase I , trihelix transcription factor, NADH dehydrogenase submit F, cycline-dependent kinase, F-box pro- tein-related, ATP synthase subunit beta, mitochondrial precursor, glucan phosphorylase putative, zinc finger family protein, heat shock protein 26, peroxiredoxin type2 putative, alcohol dehydrogenase. They could be involved in DNA, RNA and protein biosynthesis, gene transcription and transposition, intron splicing, cell signal transduction and cell structure orga-nization, defence response and energy metabolism.