抗白粉病小麦近等基因系DNA甲基化的MSAP分析

应用甲基化敏感扩增多态性（MSAP）技术检测小麦抗白粉病代换系6A／6V与感病品系京411的近等基因系NILs，及其亲本6A／6V和京411的基因组DNA甲基化变化．结果表明，NILs的整体甲基化水平（22．9％）略高于6A／6V（21．2％），低于京411（23．4％）．分析其甲基化模式发现，NILs相对京411甲基化水平降低条带比例（2．89％）明显高于甲基化提高条带（0．55％），而相对抗病亲本6A／6V，NILs甲基化水平降低条带比例（2．96％）明显低于甲基化提高条带（4．20％），说明NILs的整体基因表达水平低于6A／6V，高于京411．

DNA methylation analysis of near-isogenic lines (NILs) of wheat resistant to powdery mildew based on MSAP analysis

To investigate the DNA methylation pattern, the methylation-sensitive amplified polymorphism （MSAP） technique was used to detect the genomic DNA methylation changes of powdery mildew resistant substitution lines 6A/6V, susceptible strains of Jing 411 and its near isogenic lines （NILs）. The results showed that the total methylation level of NILs （22.9%） was slightly higher than 6A/6V （21.2%）, and the methylation level was lower than Jing 411 （23.4%）. Furthermore, the methylation pattern revealed that the percentage of demethylation bands（2.89%） in NILs was higher than that of hypermethylation bands （0.55%） in contrast with Jing 411, while the demethylation level （2.96%） in NILs was lower than hypermethylation level （4.20%） in contrast with the relative resistant parent 6A/6V. In summary, it can be con-cluded that the overall gene expression levels in NILs were lower than 6M6V and higher than Jing 411.