Chromate reduction in Streptomyces |
| |
| Authors: | S Das A L Chandra |
| |
| Institution: | Department of Microbiology, Bose Institute, Calcutta, India. |
| |
| Abstract: | Streptomyces species 3M grew in peptone yeast extract medium with 1000 micrograms/ml K2Cr2O7. Incubation of the chromate with different cell fractions in the presence of NADH and NADPH resulted in a decrease of Cr6+ in the reaction mixture. The level of Cr6+ was reduced by 82.7% by a particulate cell fraction obtained by centrifugation at 105,000 x g for 1 h, in the presence of NADH. The reducing enzyme was associated with this cell fraction. The enzyme was constitutive and reduced Cr6+ to Cr3+. |
| |
| Keywords: | |
|
|
