保肝颗粒对大鼠酒精性肝损伤的早期干预作用

观察保肝颗粒对大鼠酒精性肝损伤的早期干预作用。将144只大鼠随机分为空白组、模型组、联苯双酯组、保肝颗粒低、中、高剂量组,给药1 h后除空白组外,其余各组按10 mL/kg灌胃50%乙醇,1 h后重复灌胃50%乙醇一次,空白组灌胃等体积蒸馏水,连续6 d。于实验开始的第2、3、4、6天每组分别随机处死大鼠6只,腹主动脉取血,检测血清中丙氨酸氨基转移酶(alanine aminotransferase, ALT)、天门冬氨酸氨基转移酶(aspartate aminotransferase, AST)、碱性磷酸酶(alkaline phosphatase, ALP)、总胆红素(total bilirubin, TBIL)、肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、白介素-6(interleukin-6, IL-6)、α-谷胱甘肽-S-转移酶(α-glutathione S-transferase, α-GST)、谷氨酸脱氢酶(glutamate dehydrogenase, GLDH)、精氨酸酶1(arginase 1, Arg-1)、嘌呤核苷酸磷酸...

Early Intervention Effect of Baogan Granules on Alcoholic Liver Injury in Rats

To observe the early intervention effect of Baogan Granules on alcoholic liver injury in rats. 144 rats were randomly divided into blank group, model group, biphenyl group, Baogan granules low, medium and high dose groups. After 1 h of administration, except for the blank group, the other groups were given 50 % ethanol by gavage at 10 mL/kg, 50 % ethanol was gavaged repeatedly 1 h later, and the blank group was gavaged with the same volume of distilled water for 6 days. On the day 2, 3, 4 and 6 of the experiment, 6 rats in each group were randomly killed, and blood was collected from abdominal aorta. The contents of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TBIL), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), α-glutathione S-transferase (α-GST), glutamate dehydrogenase (GLDH), Acidase 1 (Arg-1) and purine nucleotide phosphorylase (PNP) in serum and the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in liver homogenate were determined. The results showed that compared with blank group, the serum levels of AST, ALT, ALP, TBIL, TNF-α, IL-6, α-GST, GLDH, Arg-1 and PNP in model group were significantly increased (P<0.05, P<0.01), the level of MDA in liver tissue was significantly increased, and the level of SOD was significantly decreased (P<0.05, P<0.01); Compared with model group, Baogan Granules could significantly reduce the serum levels of AST, ALT, TBIL, ALP, TNF-α, IL-6, α-GST, GLDH, Arg-1, PNP (P<0.05, P<0.01); The activity of SOD in liver tissues increased and the content of MDA decreased (P<0.05, P<0.01). Baogan granules has a good effect on liver protection in the early intervention of alcoholic liver injury in rats.