Effects of low temperatures on survival of frozen-thawed mouse embryos |
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Authors: | H Miyamoto T Ishibashi |
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Institution: | (1) Department of Animal Science, College of Agriculture, Kyoto University, 606 Kyoto, Japan |
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Abstract: | Summary Almost no damage to mouse morulae was observed between 0 and –40°C, and freezing damage to embryos in DMSO, glycerol or ethylene glycol occurred after exposure to –60, –50 or –50°C, respectively. Cooling embryos in DMSO or glycerol to temperatures below –50 to –60°C increased freezing damage. To the contrary, in the presence of ethylene, glycol, no more damage occurred after exposure to temperatures below –50°C. |
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