| 葡萄籽原花青素对营养肥胖模型大鼠肠道菌群的影响 |
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| 引用本文： | 宋雪琳,李雅梅,肖俊松,吴 华,曹雁平.葡萄籽原花青素对营养肥胖模型大鼠肠道菌群的影响[J].北京工商大学学报(自然科学版),2015,33(5):39-46. |
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| 作者姓名： | 宋雪琳 李雅梅 肖俊松 吴 华 曹雁平 |
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| 作者单位： | 北京工商大学 北京市食品风味化学重点实验室/北京市食品添加剂工程技术研究中心/ 食品质量与安全北京实验室, 北京 100048,北京工商大学 北京市食品风味化学重点实验室/北京市食品添加剂工程技术研究中心/ 食品质量与安全北京实验室, 北京 100048,北京工商大学 北京市食品风味化学重点实验室/北京市食品添加剂工程技术研究中心/ 食品质量与安全北京实验室, 北京 100048,江汉大学 生命科学院, 湖北 武汉 430070,北京工商大学 北京市食品风味化学重点实验室/北京市食品添加剂工程技术研究中心/ 食品质量与安全北京实验室, 北京 100048 |
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| 基金项目： | 国家自然科学基金资助项目(31201323)； “十二五”国家科技支撑计划项目(2011BAD23B02); 北京市属高等学校高层次人才引进与培养计划项目(0142132014)。 |
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| 摘 要： | 以高脂膳食喂养大鼠,复制营养肥胖模型,然后灌胃原花青素,采用变性梯度凝胶电泳,实时荧光定量等不依赖微生物培养的手段和多元统计分析方法,研究葡萄籽原花青素对营养肥胖模型大鼠肠道菌群生态的影响。结果表明,低剂量(100mg/(kg·体重·d))原花青素可以显著抑制大鼠肥胖,处理后大鼠肠道菌群结构与模型组分离；葡萄籽原花青素显著降低了肥胖大鼠肠道菌群中厚壁菌门的含量,提高了拟杆菌门的含量,显著降低了厚壁菌门与拟杆菌门比值；实时荧光定量检测显示,原花青素可以促进拟杆菌增殖,抑制柔嫩梭菌增殖,初步揭示出葡萄籽原花青素可能具有调节肠道菌群的功能；原花青素作用的关键微生物种属为Blautia, Bacteroides, Lactobacillus, Anaerostipes, Clostridium, Anaerofilum。
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| 关 键 词： | 葡萄籽原花青素 肥胖 肠道菌群 变性梯度凝胶电泳 实时荧光定量PCR |
| 收稿时间： | 2015/1/11 0:00:00 |
Effect of Grape Seed Proanthocyanidins on Gut Microbiota of Diet-Induced-Obesity Rats |
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| SONG Xuelin,LI Yamei,XIAO Junsong,WU Hua and CAO Yanping.Effect of Grape Seed Proanthocyanidins on Gut Microbiota of Diet-Induced-Obesity Rats[J].Journal of Beijing Technology and Business University:Natural Science Edition,2015,33(5):39-46. |
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| Authors: | SONG Xuelin LI Yamei XIAO Junsong WU Hua and CAO Yanping |
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| Institution: | Beijing Key Laboratory of Food Flavor Chemistry/Beijing Engineering and Technology Research Center of Food Additives/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048,China,Beijing Key Laboratory of Food Flavor Chemistry/Beijing Engineering and Technology Research Center of Food Additives/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048,China,Beijing Key Laboratory of Food Flavor Chemistry/Beijing Engineering and Technology Research Center of Food Additives/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048,China,College of Life Sciences,Jianghan University, Wuhan 430070,China and Beijing Key Laboratory of Food Flavor Chemistry/Beijing Engineering and Technology Research Center of Food Additives/Beijing Laboratory for Food Quality and Safety, Beijing Technology and Business University, Beijing 100048,China |
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| Abstract: | Wistar rats were fed with high fat diet to induce the diet-induced obesity model (DIO), and then gavaged with grape seed proanthocyanidins (GSP) of different doses. Culture-independent methods such as denaturing gradient gel electrophoresis (DGGE) and real-time fluorescent quantitative polymerase chain reaction (real-time PCR) were applied, coupled with multivariate analysis, to explore the effect of GSP on the microbiota of DIO rats. Results showed that GSP treatment (100mg/(kg·bw·d)) could inhibit high-fat induced obesity, and its gut microbiota profile was separated from that of the model group. Results also suggested that GSP-treated groups had the lower Firmicutes content and higher Bacteroidetes content and the ratio of Firmicutes to Bacteroidetes increased in this group. Real-time-PCR results indicated that GSP promoted the proliferation of Bacteriodes spp. and inhibited that of Clostridium leptum. The key genera the GSP might have an effect on were as follows:Blautia, Bacteroides, Lactobacillus, Anaerostipes, Clostridium, Anaerofilum. |
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| Keywords: | grape seed proanthocyanidins obesity gut microbiota denaturing gradient gel electrophoresis real time-PCR |
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