| 氨基功能化SBA-15分子筛的制备及对纤维素酶的固定化 |
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| 引用本文： | 刘路宽,苏忠亮,李露,于世涛.氨基功能化SBA-15分子筛的制备及对纤维素酶的固定化[J].青岛化工学院学报(自然科学版),2013(6):579-584. |
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| 作者姓名： | 刘路宽 苏忠亮 李露 于世涛 |
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| 作者单位： | 青岛科技大学化工学院,山东青岛266042 |
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| 基金项目： | 国家自然科学基金项目（30900126,31070520）. |
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| 摘 要： | 采用共缩聚法制备了氨基功能化SBA-15介孔分子筛,以此为载体,戊二醛作交联剂,进行了纤维素酶固定化.考察了戊二醛浓度、给酶量、pH、固定化时间等因素对固定化的影响,确定了固定化最佳条件,并对固定化酶的酶学性质进行了研究.结果表明,固定化酶最适作用温度60℃,最适作用pH为4;固定化酶Km值3.61 mg·mL-1;固定化酶的热稳定性、操作稳定性、贮存稳定性均明显高于游离酶.
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| 关 键 词： | 氨基功能化SBA-15分子筛 纤维素酶 固定化 |
Immobilization of Cellulase on Amino-functionalized SBA-15 Molecular Sieve |
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| LIU Lu-kuan,SU Zhong-liang,LI Lu,YU Shi-tao.Immobilization of Cellulase on Amino-functionalized SBA-15 Molecular Sieve[J].Journal of Qingdao Institute of Chemical Technology(Natural Science Edition),2013(6):579-584. |
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| Authors: | LIU Lu-kuan SU Zhong-liang LI Lu YU Shi-tao |
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| Institution: | 1.College of Chemical Engineering, Qingdao University of Science and Technology, Qingdao 266042, China;) |
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| Abstract: | Amino-functionalized SBA-15 mesoporous molecular sieve was prepared by the co-condensation method.The immobilization of cellulase was studied using the obtained molecular sieve as carrier and glutaraldehyde as crosslink agent.The effects of factors,such as glutaraldehyde concentration,enzyme dosage,pH and immobilization time,on immobilization were investigated.The properties of immobilized cellulase were also studied.The results showed that the optimum conditions were temperature 60 ℃,pH=4,Km=3.61 mg · mL-1,and the thermal,operational and storage stabilities of immobilized cellulase were better than those of free cellulase. |
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| Keywords: | amino-functionalized SBA-15 molecular sieve cellulase immobilization |
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