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石刁柏花药离体培养
引用本文:胡世宽,刘品华.石刁柏花药离体培养[J].河北省科学院学报,1990(2):74-78.
作者姓名:胡世宽  刘品华
作者单位:河北省科学院生物研究所,河北省科学院生物研究所,河北省科学院生物研究所,河北省科学院生物研究所,河北省外贸粮油进出口公司,河北省外贸粮油进出口公司
摘    要:诱导石刁柏花药产生愈伤组织的频率高低,同培养基中2.4—D浓度、花药低温预处理因素相关;诱导愈伤组织分化芽点获得胚性细胞后,转入N_6或MS培养基上,均可获得理想的增苗效果;石刁柏花粉试管苗在繁苗或生根培养基中根芽不能同步发生,生根需要专用培养基。

关 键 词:石刁柏  花药培养

STUDIES ON ANTHERS CULTURE OF ASPARAGUS OFFICINALIS IN VITRO
Hu Shikuan Sun Jing Ma Guirong Duan Pufan Liu Pinhua Liu Guoyu.STUDIES ON ANTHERS CULTURE OF ASPARAGUS OFFICINALIS IN VITRO[J].Journal of The Hebei Academy of Sciences,1990(2):74-78.
Authors:Hu Shikuan Sun Jing Ma Guirong Duan Pufan Liu Pinhua Liu Guoyu
Institution:Hu Shikuan Sun Jing Ma Guirong Duan Pufan Liu Pinhua Liu Guoyu
Abstract:The frequency of inducing anthers of asparagus officinalis to form callus was mainly correlative with two factors: pretreatment of anthers with low temperature and the concentration of 2.4-D in the media. At first callus of asparagus officinalis were induced to form buds and embryonic cells, then transfered to N_6 or MS media. The effect of preliferation was ideal. In anthers culture, the roots and buds of asparagus officinal could not occur synchronously. The medium for rooting was a special one.
Keywords:Asparagus officinalis Anthers culture
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