番茄黄化曲叶病毒病抗性材料的筛选鉴定

 为评估新培育番茄栽培品种对番茄黄化曲叶病毒（tomato yellow leaf curl virus，TYLCV）的抗性水平，更全面地了解这些品种的抗病性差异，以金棚1 号和金曼为感病对照，采用带毒烟粉虱自然传毒方式，对各品种的发病时间、发病率及病情指数等参数进行比较，结合PCR 及ELISA 对TYLCV 的检测结果，综合分析了18 个番茄品种对番茄黄化曲叶病毒的抗病性。结果表明，不同品种对TYLCV 的抗性差异较大。金棚1 号和金曼两份材料发病率都达到100%，病情指数在65.2 以上，属于典型的感病品种；秋光15-6、PC-88 和秋光69 号3 份材料发病率和病情指数均为0，属于抗性较高的品种；其他13 份材料的发病率和病情指数分别在5.6％～45.2%和2.6～18.0 之间，分别表现了不同程度的抗、耐病水平。

Screening and Identification of Tomato Anti-virus Variety Against Tomato Yellow Leaf Curl Virus

To assess the resistance level of the new anti-virus tomato variety against tomato yellow leaf curl virus, the time of the virus affection, the disease incidence and the disease index of 18 tomato varieties are recorded in the field by the natural transmission with Jinpeng 1 and Jinman as the susceptible control. The resistance level is evaluated by PCR and ELISA. It is shown that the resistance varies significantly among the 18 tomato varieties. Two cultivars of Jinpeng1and Jinman are very susceptible, the disease incidences both reach 100% and the disease indices are both above 65.2. Three cultivars of Qiuguang 15-6, PC-88, and Qiuguang 69 show a high level resistance, the disease incidences and the disease indices are all 0. The rest 13 cultivars show different degrees of resistance levels, the disease incidences and the disease indices are between 5.6% and 45.2%, and 2.6 and 18.0, respectively.