银杏叶提取物与人参皂苷对血管活性物质的影响

银杏叶提取物(GBE)和人参皂苷(GS)均具有显著的心血管药理活性,但未见二者配伍应用于心血管疾病治疗的研究和报道.采用血清药理学方法研究GBE与GS单独和配伍对氧化损伤内皮细胞代谢一氧化氮(NO)、内皮素(ET-1)及丙二醛(MDA)的影响.GBE、GS均可升高氧化损伤内皮细胞NO的代谢水平,降低其ET-1和MDA的代谢水平.二者配伍后抗脂质过氧化的作用增强,但对氧化损伤内皮细胞NO代谢的调节作用不及GBE和GS,分别降低了29.9%和66.7%,差异显著(P＜0.05);对ET-1代谢的调节作用虽优于GBE,但与GS相比降低了11.6%.这提示GBE和GS配伍后对内皮细胞血管活性物质NO和ET-1的调节作用未起到协同效果,GBE和GS配伍能否应用于心血管疾病的治疗还是一个值得探讨的课题.

Effect of Ginkgo biloba extract and ginsenosides on the Vascular Activity Substance production in endothelium cells

Though Ginkgo biloba extract(GBE)and ginsennosides(GS)also have notability bioactivity on cardiovascular disease,there is little literature about applying the compound of them to treat cardiovascular disease.After endothelium cells suffering from oxidative injury were treated with serum containing GBE,GS and the compound of them(GG),respectively,production of NO,ET and MDA in them was observed by applying serum pharmacology.Both GBE and GS up-regulate NO level and down-regulate ET-1 and MDA level in endothelium cells suffering from oxidative injury.Anti-oxidation activity increased after GBE and GS were combined.But the NO up-regulation of GG was inferior to that of GBE and GS,which decreased by 29.9% and 66.7% when compared with that of GBE and GS,respectively.The ET-1 down-regulation of GG was superior to that of GBE, but decreased by 11.6% compared with that of GS.The compatibility of GBE and GS do not show synergic effect on NO and ET-1 regulation in endothelium cells and it is valuable to discuss that the compound of GBE and GS is used to treat cardiovascular diseases.