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Cloning of theAspergillus awamori glucoamylase gene and expression inSaccharomyces cerevisiae |
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| Authors: | Yongji Wang Hongdi Liu Tong Sun Shuzheng Zhang |
| Institution: | WANG Yongji, LIU Hongdi *, SUN Tong and ZHANG Shuzheng Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. * Corresponding author. |
| Abstract: | The A. awamori glucoamylase I gene was obtained by RT_PCR and inserted into the plasmid pAC1. We constructed an expression vector pAC1_GA, which was used to transform the yeast S. cerevisiae AS 2 1364 without auxotrophic marker. The transformant secreted glucoamylase into the medium efficiently and degraded starch. The glucoamylase activity of the culture filtrate is up to 8 4 U/mL. |
| Keywords: | Aspergillus awamori glucoamylase gene cloning and expression Saccharomyces cerevisiae |
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