异丙肾上腺素诱发大鼠特异性心肌缺血所引起心肌细胞病理组织的变化

目的 观察皮下注射不同天数异丙肾上腺素(1 mg/kg体质量)诱发大鼠特异性心肌病所引起心肌组织切片HE染色、VEGF(血管内皮生长因子)、FGF(成纤维细胞生长因子)表达及心电图T波的变化。方法 除空白组外各组大鼠皮下多点注射异丙肾上腺素(1 mg/kg)，分别为连续皮下多点注射异丙肾上腺素(1 mg/kg)2 d组、连续皮下多点注射异丙肾上腺素(1 mg/kg)4 d组、连续皮下多点注射异丙肾上腺素(1 mg/kg)6 d组。给药1周后，大鼠麻醉，测心电图，取心脏，采用HE染色观察大鼠心肌细胞损伤程度的变化，采用免疫组化技术染色观察心肌组织切片VEGF(血管内皮生长因子)、FGF(成纤维细胞生长因子)表达的变化。结果 与空白对照组相比连续皮下多点注射异丙肾上腺素(1 mg/kg)6 d组HE染色组织切片镜下观察具有明显差异，同时随着造模时间的延长模型组心肌组织切片VEGF(血管内皮生长因子)、FGF(成纤维细胞生长因子)表达情况与空白对照组比也存在显著性差异。结论 本研究结果表明与空白对照组相比连续皮下多点注射异丙肾上腺素(1 mg/kg)6 d组模型大鼠特异性心肌病所引起的心肌组织切片HE染色、VEGF(血管内皮生长因子)、FGF(成纤维细胞生长因子)表达及心电图T波的变化具有显著性差异，因此大鼠连续皮下多点注射异丙肾上腺素(1 mg/kg)6 d可以作为特异性心肌病药效评价的实验动物模型使用。

Pathological Changes of Specific Myocardial Ischemia Inducedby Isoproterenol in Rats Cardiomyocytes

Objective To observe the changes of HE staining, VEGF and FGF expression in rat myocardial tissue slices induced by subcutaneous injection of isoproterenol (1 mg/kg) in different days. Method Except for the natural control group, rats were injected subcutaneously with isoproterenol (1 mg/kg) 2-day group、4-day group， 6-day group. One week after injection, the rats were anesthetized, the ECG was measured, and the heart was taken. HE staining was used to observe the changes of rat myocardial cell damage. Immunohistochemical staining was used to observe the changes of VEGFand FGF expression. Result Compared with the natural control group, continuous subcutaneous injection of isoproterenol (1 mg/kg) at 6-day group showed significant differences in HE staining. Expression of VEGF (vascular endothelial growth factor) and FGF (fibroblast growth factor) in the model group was also significantly different from that in the natural control group. Conclusion Rats were injected subcutaneously with isoproterenol (1 mg/kg) 6 days can be used as an experimental animal model for evaluating the efficacy of heterosexual cardiomyopathy.