一种快速取大鼠膝关节滑膜的方法

目的 建立一种较传统方法更为快速可行地获取大鼠膝关节滑膜组织的方法。方法 通过查阅文献了解取大鼠膝关节滑膜组织传统方法，并查阅大量大鼠膝关节解剖图谱，在实验中随机将60只造模成功的佐剂性关节炎实验大鼠分成两个小组。30只大鼠采用传统方法取大鼠膝关节滑膜组织，另外30只大鼠采用新方法取大鼠膝关节滑膜组织。结果 传统方法30只大鼠中23只取出较为完整的滑膜组织。新方法30只大鼠全部完整取出膝关节滑膜组织；传统方法平均取一只大鼠单侧膝关节滑膜组织耗时为(15.26±3.41)min，新方法平均每只耗时(10.12±2.34)min，两者比较有显著差异(P<0.01)。结论 新方法较传统方法能更为快速可行地取出大鼠膝关节滑膜组织。

A Rapid Method for Removing Synovial Membrane of Rat Knee Joint

Objective To study a more rapid and feasible method than the traditional method for removing knee joint synovial tissue of rats. Method The traditional method of extracting synovial tissue of the rat knee joint were studied by consulting the literature, and a large number of anatomical maps of the knee joint of the rats were examined. In the experiment, 60 successful adjuvant arthritis rats were randomly divided into two groups: the synovial tissue of the knee joint of 30 rats and the synovial tissue of the knee joint of the other 30 rats were taken from the synovial tissue of the knee joint of the rats by the traditional method , and the synovial tissue of the knee joint of the other 30 rats was obtained by a new method . Result Twenty-three rats were taken out of the synovial tissue in the traditional method . The synovial tissue of knee joint was taken out completely in 30 rats by the new method , and the average time consuming of unilateral synovial tissue was about (15.26±3.41) min in the traditional method, and (10.12 ±2.34) minutes in the new method . There was a significant difference between the two method (P<0.01). Conclusion The new method is more rapid and feasible than the traditional method in removing synovial tissue of rat knee joint.