| 铁钉菜抗生物质的初步提取及检测 |
| |
| 引用本文： | 张迪,刘艳如,郑怡,林勇. 铁钉菜抗生物质的初步提取及检测[J]. 福建师范大学学报(自然科学版), 2009, 25(3) |
| |
| 作者姓名： | 张迪 刘艳如 郑怡 林勇 |
| |
| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350108
2. 福建农业科学院土壤肥料研究所,福建,福州,350013 |
| |
| 基金项目： | 福建省科技厅重点项目 |
| |
| 摘 要： | 对铁钉菜(Ishige okamura)的乙醚和乙醇总提取物及其不同极性的萃取物的抗生活性进行了测定,包括对细菌、真菌及藻类的抑制作用.结果表明,铁钉菜的乙醚、乙醇总提取物具有较高的抑制细菌活性,对海洋小球藻(Chlorella spp)和球形棕囊藻(Phaeocytis globosa)亦有良好的抑制作用.铁钉菜不同极性溶剂的萃取物表现出不同的抗生活性,其中氯仿相抑制细菌的能力最高,正丁醇相对真菌菌丝的抑制效果最好,而有抑藻活性的成分则主要集中在低极性的石油醚相和氯仿相中,氯仿相对供试细菌生长的最小抑菌质量浓度为3.125～12.500 mg/mL.采用薄层层析分离氯仿组分得到6个条带,其中的Rf0.79带的抑菌效果最好.
|
| 关 键 词： | 铁钉菜 抑菌 抑藻 薄层层析 |
Extraction and Determination of Antibiotic Substance from Ishige okamurai |
| |
| ZHANG Di,LIU Yan-ru,ZHENG Yi,LIN Yong. Extraction and Determination of Antibiotic Substance from Ishige okamurai[J]. Journal of Fujian Teachers University(Natural Science), 2009, 25(3) |
| |
| Authors: | ZHANG Di LIU Yan-ru ZHENG Yi LIN Yong |
| |
| Affiliation: | 1.College of Life Sciences;Fujian Normal University;Fuzhou 350108;China;2.Institute of Soil and Fertilizer Fujian Academy of Agricultural Sciences;Fuzhou 350013;China |
| |
| Abstract: | The ethanol-ether crude extract and different extractive fractions of Ishige okamura were tested for their antibiotic activities against some fungi,bacteria and algae.The results showed that crude extract had a good antibacterial activity and effective resistance to the growth of Phaeocytis globosa and Chlorella spp.There were different antibiotic activities in extractive fractions with different solvents.Chloroform extract had the strongest inhibition to bacteria,n-butyl alcohol extract had the strongest r... |
| |
| Keywords: | Ishige okamurai antimicrobial antialgal TLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
