| 环境细菌遗传多样性分析的基因标签串测序法 |
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| 引用本文： | 杨官品. 环境细菌遗传多样性分析的基因标签串测序法[J]. 湖北大学学报(自然科学版), 1999, 21(3): 291-293 |
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| 作者姓名： | 杨官品 |
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| 作者单位： | [1]湖北大学生命科学学院 [2]中华人民共和国农业部 |
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| 基金项目： | 湖北省自然科学基金资助项目，武汉市科学技术委员会晨光计划资助项目 |
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| 摘 要： | 把研究真核细胞表达基因种类和水平的基因标签串测序法引入环境细菌遗传多样性分析，选择细菌６Ｓ核糖体ＲＮＡ基因（ｒＤＮＡ）内０２６碱基对高变异区段为该基因变异类型的标签，连接成标答中并测序，用标签类型、频率和２多样性指数等参数在分子水平上分析环境细菌遗传多样性，建立了环境细菌遗传多样性分析的基因标签串测序法，该方法在分析环境隐含遗传多样性、提示污染生物学效应和评价环境质量等方面有的应用价值。因使用共同
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| 关 键 词： | 环境细菌 遗传多样性 DNA 基因标签串测序 |
| 修稿时间： | 1998-12-20 |
Gene Tag Serial Sequencing of Environmental Bacterial Diversity Analysis |
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| Yang Guanpin Huang Hong Liu Yingjie Zhang Kai. Gene Tag Serial Sequencing of Environmental Bacterial Diversity Analysis[J]. Journal of Hubei University(Natural Science Edition), 1999, 21(3): 291-293 |
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| Authors: | Yang Guanpin Huang Hong Liu Yingjie Zhang Kai |
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| Affiliation: | Yang Guanpin 1 Huang Hong 1 Liu Yingjie 2 Zhang Kai 2 |
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| Abstract: | Simple morphological character and the failure of the major portion of bacteria in surviving artificial cultivation have hindered bacterial diversity from analysis through traditional taxonomic approaches.Gene tag serial sequencing was developed in this study by using a highly variable fragment of 16S ribosomal RNA(rDNA) gene as variant representing tags in order to assaying environmental bacterial diversity at molecular level.The technigue holds potential applications in detecting hidden genetic diversity,tracing biological effectiveness of pollutants and evaluating environmental quality.In addition,common tag dependent data from different sources can be integrated each other,which will greatly facilitate joint researches. |
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| Keywords: | Environmental bacteria Genetic diversity 16S ribosomal RNA (rDNA) gene Gene tag serial sequencing |
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