| 免疫核糖核酸作用机制的研究──抗SRBCIRNA的电泳分离及分离组分对靶细胞的作用 |
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| 引用本文： | 郑昌学,Shukang Hu,Bosco S. Wang,John A.Mannick,Eloise Heacock.免疫核糖核酸作用机制的研究──抗SRBCIRNA的电泳分离及分离组分对靶细胞的作用[J].清华大学学报(自然科学版),1988(6). |
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| 作者姓名： | 郑昌学 Shukang Hu Bosco S. Wang John A.Mannick Eloise Heacock |
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| 作者单位： | 清华大学生物科学技术系，美国氰化物公司 Lederle实验室，美国氰化物公司 Lederle实验室，美国哈佛医学院外科系，美国哈佛医学院外科系 |
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| 摘 要： | 报道了用聚丙烯酰胺凝胶电泳法和电泳洗脱法分离抗 ＳＲＢＣ ＩＲＮＡ及各分离组分的 活性．结果表明电泳能将ＩＲＮＡ分为３０多条带．沉降系数为８～１８ｓ的Ｆｒ２组分具有传递免疫反应的活性。测定了总ＩＲＮＡ及其各组分对 Ｔ细胞和 Ｂ细胞的作用，表明总 ＩＲＮＡ既能激活Ｔ细胞，亦能激活Ｂ细胞． Ｆｒ２－ａ能激活 Ｔ细胞，Ｆｒ２－２，Ｆｒ２－ｂ、 Ｆｒ２－ｃ和Ｆｒ２－ｄ都能激活Ｂ细胞、实验还表明ＩＲＮＡ不大可能作为抗体的直接模板。
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| 关 键 词： | 免疫核糖核酸 凝胶电泳 T细胞 B细胞 绵羊红血球 |
Studies on Mechanism of Action of Immune RNA -Fractionation of IRNA by Polyacrylamidc Gel Electrophoresis and Effects of the Fractions on Target Cells |
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| Zheng Changxue.Studies on Mechanism of Action of Immune RNA -Fractionation of IRNA by Polyacrylamidc Gel Electrophoresis and Effects of the Fractions on Target Cells[J].Journal of Tsinghua University(Science and Technology),1988(6). |
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| Authors: | Zheng Changxue |
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| Abstract: | Fractionation of IRNA against SRBC by polyacrylamide gel electrophoresis showed that there were more than 30 RNA components in the IRNA preparation. The fractions of IRNA were collected from the gel by electrophoretic elution, and the activity of transferring immune response of the fractions was detected.The results showed.that only Fr-2 with sedimentation coefficient of 8 ~18 8 is active. The biological effects of total IRNA and their fractions on T cell and B cell were observed.The results showed that total IRNA is active in stimulating both T cell and B cells,while Fr2-a stimulates only T cells,and Fr-2a,b,c, d stimulates B cells.The Fr-2c is more active in B cellstimulation, Theexperiments also showed that it is unlikely for IRNA to act as the template in antibody synthesis. |
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| Keywords: | immune RNA gel electrophoresis T cell B cell SRBC |
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