| 高通量筛选污水中的硝化细菌 |
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| 引用本文： | 赵翠娟,宋文军,朱高雄,魏纪平,李博志,宋罐兰,闫春燕,龚忱.高通量筛选污水中的硝化细菌[J].天津师范大学学报(自然科学版),2013(2):94-96. |
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| 作者姓名： | 赵翠娟 宋文军 朱高雄 魏纪平 李博志 宋罐兰 闫春燕 龚忱 |
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| 作者单位： | [1]天津商业大学生物技术与食品科学学院,天津300134 [2]天津市兴源环境技术工程有限公司,天津300384 [3]天津清鉴生物科技有限公司,天津300384 |
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| 基金项目： | 天津市滨海新区科技创新专项资金自主创新重大项目（2011-BK120042） |
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| 摘 要： | 为了降低污水中氮元素对环境的污染,利用分离培养基从污水中分离出94株单菌,对单菌进行富集,再利用鉴定培养基、氨氮、亚硝酸盐和硝酸盐快速测定试剂盒初筛出41株硝化细菌,通过功能验证复筛出25株高效的硝化细菌.这25株硝化细菌均可将市政污水中的COD降解到40~60 mg/L,符合污水的COD一级降解标准,氨氮的降解率为70%~90%,也符合污水中氨氮的降解标准.
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| 关 键 词： | 高通量 筛选 污水 硝化细菌 |
High-throughput screening nitrifying bacteria in sewage |
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| ZHAO Cuijuan,SONG Wenjun,ZHU Gaoxiong,WEI Jiping,LI Bozhi,SONG Guanlan,YAN Chunyan,GONG Chen.High-throughput screening nitrifying bacteria in sewage[J].Journal of Tianjin Normal University(Natural Science Edition),2013(2):94-96. |
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| Authors: | ZHAO Cuijuan SONG Wenjun ZHU Gaoxiong WEI Jiping LI Bozhi SONG Guanlan YAN Chunyan GONG Chen |
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| Institution: | 1 ( 1. College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China; 2. Tianjin Spring Environmental Technology ＆ Engineering Co., Ltd., Tianjin 300384, China; 3. Tianjin Qingiian Bio-Technology Co., Ltd., Tianjin 300384, China) |
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| Abstract: | In order to reduce the nitrogen pollution in sewage to environment, 94 bacteria were isolated from sewage by isolation medium. These bacteria were enriched, and 41 nitrifying bacteria were screened through identification medium, ammonia, nitrite and nitrate kit. 25 efficient nitrifying bacteria were rescrecned according to their different function, resulted in that the COD reduced to 40-60 mg/L in the municipal wastewater, which met the primary degradation standard of COD, the ammonia nitrogen degradation rate reached 70%-90%, which met the degradation standard of ammonia nitrogen in the sewage, too. |
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| Keywords: | high-throughput screen sewage nitrifying bacteria |
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