Survival of rabbit embryos after rapid freezing and thawing |
| |
Authors: | V. H. Rao I. V. Mongha M. R. Ansari N. K. Bhattacharyya |
| |
Affiliation: | (1) Indian Veterinary Research Institute, 243 122 Izatnagar, (India) |
| |
Abstract: | Summary Frozen storage of rabbit embryos at the 16-cell stage in 2.0 M dimethylsulfoxide (DMSO) in phosphate-buffered saline (PBS) was achieved by a 2-step procedure. After storage for 10 days at–196°C they were revived by rapidly thawing at 500°C/min. On transfer of these embryos to pseudopregnant foster mothers, 50% survived to term. The difference in in vivo survival between frozen-thawed and frozen-thawed-cultured embryos was not significant.Acknowledgment. The authors thank the Director for the facilities. VHR is supported by Council of Scientific and Industrial Research (India). |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |