| 胆固醇氧化酶高产菌株的诱变选育及产酶条件优化 |
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| 引用本文： | 黄寅,葛飞,陶玉贵,汤斌,李英.胆固醇氧化酶高产菌株的诱变选育及产酶条件优化[J].安徽工程科技学院学报,2009,24(4):8-11. |
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| 作者姓名： | 黄寅 葛飞 陶玉贵 汤斌 李英 |
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| 作者单位： | 1. 安徽工程科技学院,生化工程系,安徽,芜湖,241000;安徽工程科技学院,微生物发酵安徽省工程技术研究中心,安徽,芜湖,241000
2. 安徽工程科技学院,生化工程系,安徽,芜湖,241000 |
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| 基金项目： | 安徽高校自然科学基金资助项目，安徽工程科技学院引进人才科研启动基金资助项目，芜湖市科技计划基金资助项目 |
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| 摘 要： | 在对细脚拟青霉、玫烟色拟青霉、粉拟青霉、香菇、平菇、金针菇等胆固醇氧化酶产生菌初筛的基础上,选择以产酶较高的玫烟色拟青霉作为紫外诱变的出发菌株,筛选出一株相对高产突变菌株MFEC006,其酶活达到了0.548 3 U/mL,比出发菌株提高了154%,经8次传代培养,突变株性质稳定.对MFEC006菌株产酶条件进行优化,得出其最佳产酶条件为:pH为6.5.温度27℃,接种量10%,摇床转速180 r/min.
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| 关 键 词： | 胆固醇氧化酶 玫烟色拟青霉 紫外诱变 |
Screening of high-yield strains of cholesterol oxidase by ultraviolet mutation and optimization of fermentation conditions |
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| HUANG Yin,GE Fei,TAO Yu-gui,TANG Bin,LI Ying.Screening of high-yield strains of cholesterol oxidase by ultraviolet mutation and optimization of fermentation conditions[J].Journal of Anhui University of Technology and Science,2009,24(4):8-11. |
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| Authors: | HUANG Yin GE Fei TAO Yu-gui TANG Bin LI Ying |
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| Institution: | HUANG Yin1,2,GE Fei1,TAO Yu-gui1,TANG Bin1,LI Ying1(1.Dept.of Bioch.Engn.,Anhui University of Technology , Science,Wuhu 241000,China,2.Anhui Engineering Technology Research Center of Microbial Fermentation,China) |
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| Abstract: | On the basis of screening of Paecilomyces tenuipes,Paecilomyces fumosoroseus,Paecilomyces farinosus,Pleurotus ostreatus,Lentinus edodes and Flammulina velutipes,this paper chooses Paecilomyces fumosoroseus as original strain of UV treatment.The cholesterol oxidase producing mutant strain MFEC006 was obtained from Paecilomyces fumosoroseus,the enzyme activity reached 0.548 3 U/mL,154% higher than that of the starting strain.The properties of the mutant strain were stable after eight generations.The optimal f... |
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| Keywords: | cholesterol oxidase paecilomyces fumosoroseus ultraviolet mutation |
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