Prophylaxis of tumor through oral administration of IL-12 GM-CSF gene carried by live attenuated salmonella |
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Authors: | Li Yuhua Chen Hui Guo Kunyuan Xie Yongmei Wang Feng Xie Kuangcheng Huang Jiangsheng Ren Daming |
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Institution: | (1) Department of Hematology, Zhujiang Hospital, First Military Medical University, 510282 Guangzhou, China;(2) Institute of Genetics, School of Life Science, State Key Laboratory of Genetic Engineering, Fudan University, 200433 Shangshai, China;(3) Central Research Lab of First Hospital of Shanghai, 200000 Shangshai, China |
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Abstract: | A live attenuated AraA- autotrophic mutant ofSalmonella typhimurium (SL3261) was used as carrier for eukaryotic expression vectors EGFPN1, pCMVmIL-12, pCMVhIL-12, pCMVmGM-CSF and pCMVhGM-CSF
and was administered orally to BALB/c and C57BL/6 mice. After 6 weeks, these mice were challenged with 4T1 and Lewis tumor
cells respectively. GFP expression and gene integration could be detected in mice’s livers, spleens, intestines, kidneys and
tumors. The serum level of cytokines increased significantly in treated mice, so did the ratio of CD
8
+
/CD
4
+
, which resulted in the tumor regression and prolongation of the survival time of those mice. These researches laid an experimental
foundation for the tumor gene therapy using live attenuated salmonella. |
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Keywords: | oral administration gene therapy attenuated salmonella |
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